多光子激发随机扫描显微成像软件系统仿真Simulation of Software for Multi-photon Random Access Scanning Microscopy
张红民;易秋实;李德荣;骆清铭;曾绍群;
摘要(Abstract)：
多光子激发随机扫描显微成像系统将为神经功能成像提供一个有效工具而成为研究热点,但成像软件系统的研制是其难点之一。分析了多光子激发随机扫描显微成像的实验流程,在VC6.0开发平台下,对飞秒激光扫描控制和荧光信号采集,全视场图像重构,感兴趣区域选取,图像和信号曲线动态同步显示等主要功能进行了软件仿真,对开发完整的多光子激发随机扫描显微成像软件系统具有重要的参考价值。
关键词(KeyWords)： 随机扫描;显微成像;仿真
基金项目(Foundation): 国家科技攻关计划资助项目(编号:2005BA711A04);; 国家自然科学基金资助项目(编号:30370463,30328014)
作者(Author): 张红民;易秋实;李德荣;骆清铭;曾绍群;
Email:
DOI:
参考文献(References)：
- 1.W R Zipfel,R M Williams,W W Webb.Nonlinear magic:multiphoton microscopy in the biosciences[J].Nat Biotechnol,2003;21(11):1369～1377
- 2.F Helmchen,W Denk.Deep tissue two-photon microscopy[J].Nat Me-thods,2005;2(12):932～940
- 3.S Q Zeng,X H Lv,C Zhan et al.Simultaneous compensation for spatial and temporal dispersion of acousto-optical deflectors for two-dimen-sional scanning with a single prism[J].Opt Let,2006;31(8):1091～1093
- 4.X H Lv,C Zhan,S Q Zeng et al.Construction of multiphoton laser scanning microscope based on dual-axis acousto-optic deflector[J].Rev Sci Instrum,2006;77:046101
- 5.A Bullen,S S Patel,P Saggau.High-speed,random-access fluorescence microscopy:I.High-resolution optical recording with voltage-sensitive dyes and ion indicators[J].Biophys J,1997;73:477～491
- 6.V Iyer,B E Losavio,P Saggau.Compensation of spatial and temporal dispersion for acousto-optic multiphoton laser-scanning microscopy[J].Biomed Optics,2003;8(3):460～471
- 7.T A Pologruto,B L Sabatini,K Svoboda.ScanImage:Flexible software for operating laser scanning microscopes.BioMedical Engineering On-Line2,http://www.biomedical-engineering-online.com,2003
- 8.J D Lechleiter,D T Lin,I Sieneart.Multi-photon laser scanning mi-croscopy using an acoustic optical deflector[J].Biophys J,2002;83:2292～2299